Effect Of Catalase On Hydrogen Peroxide

My aim is to confab how the effect of catalase has on the head for the hillsdown of enthalpy peroxide into water system and oxygen. 2H202(aq) 2H20(l) + 02(g) My expectancy is as you increase the concentration of hydrogen peroxide the catalase will break it down faster and therefore the time will be faster The Independent variable is hydrogen peroxide I will purpose an appropriate range including the concentrations of 0. 1%, 0. 2%, 0. 5%, 1%, and 2% The dependant variable will be the time taken in seconds to measure how long the catalase takes to breakdown the hydrogen peroxide into water and oxygen.There were a few controlled variables. The amount of hydrogen peroxide used was kept at a constant 10cm3. This allows for a fair test as the strain paper would have to travel the same distance. I repeated the examine an extra 3 times, allowed me to calculate a mean time. There is perpetually a need for a repeat as it improves the reliability of the experiment done. It in like mann er allows us to remove any anomalies in our results which h gives us a good rough estimate of the average. I carried out a sui control panel control experiment to prove that it was the enzyme in fact happy chance down the hydrogen peroxide.In this experiment I boiled the enzyme catalase to denature it so it wouldnt be able to function properly. I then time-tested this enzyme by dipping the slobber paper in it and then into the concentration of 2% hydrogen peroxide. As the enzyme was denatured it could not break down the hydrogen peroxide and could not get the filter paper to rise from the substructure of the test tube. There were many run a lay on the lines with this experiment. Hydrogen peroxide is an irritant that sens cause irritation if left on skin.Boiling water is another hazard as that can cause scolding of the skin. There is also the risk of smashed glass on which anyone could cut themselves on. Another danger is the bunsen burner on which someone could quite easily burn themselves. There argon procedures in place to prevent these problems, wearing eye protection and washing hands after the use of hydrogen peroxide. Another prevention is tutelage glass wear, boiling water and the Bunsen burner away from the edge of the table to prevent anything being knocked and falling off.ANALYSISMy data shows that what I predicted to be true, as you increased the concentration of hydrogen peroxide the time taken for the filter paper to reach the top of the test tube is smaller. This data is also shown in my graphs. The reliability of my results are good as they are shown to be accurate looking at other data that has been accumulated. The accuracy of my results attend high as they correspond to other results although improvements could be made by making sure that the take aim of hydrogen peroxide is accurate and much repeats are taken.My aim was to see the how catalase would breakdown hydrogen peroxide into water and oxygen and to conclude following my d ata and results it clearly shows that my aim was precise well reached as an appropriate target. I could do further cast by changing the independent variable changing the pH. The controlled variables would be the level of hydrogen peroxide and level of water used. In this result I would notice that as the pH decreased the enzymes would denature and the breakdown of catalase would torpid down.