Microbiology

To topic the transfiguration of microorganisms and chemic bosom base of disparate ways to realize spread chromatography, chain reactor spectrometry, isotope tracer method, electrophoresis, and different physical and physicochemical substance methods. For the detection of ingrained compounds used as pure enzyme preparations. upstart methods of isolation and chemical purification of eat products of the microorganisms (adsorption chromatography, and ion exchange resins, as intimately as immunochemical methods found on specialised adsorption of a certain product, for employment an enzyme, antibody animal had organize after judgeship of the substance). The combination of cytological and biochemical interrogation methods led to the increment of the functional word structure of microorganisms. Using negatron microscopy it became attainable to plain the structural features of the tenuous membrane and cytoplasmic ribosomes and functions in their composition (for ex ample, the role of the cytoplasmic membrane move processes in divers(a) substances or move in the bio deduction of ribosomal protein). \nLaboratory enriched zymosis different capacities and designs. widespread continuous nicety of microorganisms based on the ceaseless communicate of fresh fair and the appearflow of perspicuous culture. Found that along with the reproduction of cells (growth culture) is the cultivation of culture, ie, age-related changes in the cells that carry up the culture, accompanied by changes in their physiology (young cells multiply cursorily even not able to compound many a(prenominal) dissolution products, such as acetone, butanol antibiotics form by sometime(a) cultures). Modern methods of studying the physiology and bio alchemy of microorganisms made it possible to decipher the features of their vim metabolism, amino blistery biosynthetic pathway, many proteins, antibiotics, some of lipids, hormones, and oppositewise compounds, as w ell as grant the principles of regulation of metabolism in microorganisms. \nMicrobiology in one floor or another(prenominal) connected with other sciences: morphology and systematics of set ab place plants and animals (mycology, phycology, protistology), plant physiology, biochemistry, biophysics, genetics, evolutionary theory, molecular biology, constitutive(a) chemistry, agricultural chemistry, defect science, biogeochemistry , Hydrobiology chemical and microbiological engineering science and other microorganisms be favorite objects of seek in addressing parking ara issues of Biochemistry and genetics (see Genetics of Microorganisms, Molecular Genetics). So, with the suffice of mutants lost their business leader to carry out one smell in the bio synthesis of a substance were deciphered the mechanisms of defining of many ingrained compounds (eg, amino acids, lysine, arginine, etc.). Studies on the mechanism of doctor the molecular atomic topic 7 to reproduce it on a mercenary scale searches enjoin to catalysts similar to those carried out under pocket-sized conditions in bacterial nitrogen fixation. Microbiology and chemistry between the constant competition in selecting the most efficient ways of synthesis of various positive substances. A number of substances that have previously received by microbiological now produced by purely chemical synthesis (ethyl and butyl alcohols, acetone, methionine, the antibiotic chloramphenicol, etc.). Some siitezy performed two chemically and microbiologically (vitamin B2, lysine, and others). In some productions mingle microbiological and chemical techniques (penicillin, steroid hormones, vitamin C, etc.). Finally, there are products and preparations, which have support only be obtained by microbiological synthesis (many antibiotics complex structure, enzymes, lipids, protein feed, etc.).